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miniprotean ii cell system  (Bio-Rad)


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    Structured Review

    Bio-Rad miniprotean ii cell system
    Miniprotean Ii Cell System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miniprotean+ii/Mini-PROTEAN+2-D+Cell/10__1016_slash_j__foodchem__2026__147914-92-16-15
    Average 96 stars, based on 3092 article reviews
    miniprotean ii cell system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: Molecular adjuvant and vaccine
    Article Snippet: .. The samples were applied to the gel in loading buffer (50 mM Tris-HCl pH 6.8, 100 mM DTT, 2% SDS, 0.1% bromophenol blue) and then, SDS-PAGE was performed in electrophoresis buffer (20 mM Tris-base, 67 mM glycine, 0.1% SDS) at 12 mA for 30 min and then at 120 V at 30 mA for approximately 2 hrs in a MiniProtean® II (BioRad) cell. ..

    Electrophoresis:

    Article Title: Molecular adjuvant and vaccine
    Article Snippet: .. The samples were applied to the gel in loading buffer (50 mM Tris-HCl pH 6.8, 100 mM DTT, 2% SDS, 0.1% bromophenol blue) and then, SDS-PAGE was performed in electrophoresis buffer (20 mM Tris-base, 67 mM glycine, 0.1% SDS) at 12 mA for 30 min and then at 120 V at 30 mA for approximately 2 hrs in a MiniProtean® II (BioRad) cell. ..

    Article Title: The Hamster Model for Identification of Specific Antigens of Taenia solium Tapeworms
    Article Snippet: .. Electrophoresis was carried out in a MiniProtean II (Bio-Rad) camera at 100 V. The crude extract gels were stained with Coomassie brilliant blue and the E/S Ag gels were silver-stained. .. Western Blot Assay We carried out preparative 10% SDS-PAGE using 2 μ g of protein for the crude extracts or E/S Ag and 200 ng of pure antigens for every linear millimeter of gel as well as a prestained molecular weight marker (BenchMark Prestained protein ladder, Invitrogen).

    Article Title: Identification of the Most Immunoreactive Antigens of Candida auris to IgGs from Systemic Infections in Mice.
    Article Snippet: .. The 1D electrophoresis was carried out by loading 40 μg of protein to 10% acrylamide gels and running them at 70 mA, 100W, and 200 V for 45 min in a Miniprotean II (Bio-Rad, Hercules, California, USA), using the Page Ruler Plus (Thermo Fisher Scientific) as the protein standard. .. For 2D electrophoresis, the method described by Pellon et al.23 was used, loading 800 μg of protein to 18 cm long Immobiline DryStrip gels (pH 3−10, GE Healthcare) for the isoelectric focusing (IEF), and using 10% acrylamide gels for the https://doi.org/10.1021/acs.jproteome.3c00752 J. Proteome Res.

    Staining:

    Article Title: The Hamster Model for Identification of Specific Antigens of Taenia solium Tapeworms
    Article Snippet: .. Electrophoresis was carried out in a MiniProtean II (Bio-Rad) camera at 100 V. The crude extract gels were stained with Coomassie brilliant blue and the E/S Ag gels were silver-stained. .. Western Blot Assay We carried out preparative 10% SDS-PAGE using 2 μ g of protein for the crude extracts or E/S Ag and 200 ng of pure antigens for every linear millimeter of gel as well as a prestained molecular weight marker (BenchMark Prestained protein ladder, Invitrogen).

    other:

    Article Title: Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress
    Article Snippet: Equal amounts of protein for each sample were separated by SDS-PAGE in a minigel apparatus (MiniPROTEAN II; Bio-Rad).

    Modification:

    Article Title: A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants.
    Article Snippet: Extradiol dioxygenases (EDOs) catalyze the meta cleavage of catechol into 2-hydroxymuconaldehyde, a critical step in the degradation of aromatic compounds in the environment.. In the present work, a novel thermophilic extradiol dioxygenase from Thermomonospora curvata DSM43183 was cloned, expressed, and characterized by phylogenetic and biochemical analyses.. This enzyme exhibited excellent thermo-tolerance, displaying optimal activity at 50 C, remaining >40% activity at 70 C. Structural modeling and molecular docking demonstrated that both active center and pocket-construction loops locate at the C-terminal domain.

    Western Blot:

    Article Title: Candida albicans increases the aerobic glycolysis and activates MAPK-dependent inflammatory response of liver sinusoidal endothelial cells.
    Article Snippet: .. For Western Blotting (WB), samples were loaded into 10 % SDSpolyacrylamide gels and run at 70 mA, 100 W and 200 V for 45 min in a Miniprotean II (Bio-Rad), using the Page Ruler Plus as protein standard (Thermo Fisher Scientific). .. Proteins were then transferred to Hybond-P PVDF membranes (GE Healthcare) for 1 h at 400 mA.

    Article Title: Subcellular localisation of cherry leaf roll virus coat protein and genomic RNAs in tobacco leaves.
    Article Snippet: The in vivo subcellular location of the coat protein and RNAs of cherry leaf roll nepovirus (CLRV) was studied in infected tobacco plants by two different approaches and it was correlated with the cytopathic structures induced by the virus.. Subcellular fractions were obtained by differential centrifugation, visualised by electron microscopy and analysed for their viral RNA and coat protein content by Northern blot and Western blot analysis, respectively.. Results indicate that viral RNAs accumulated preferentially at the microsomal fraction.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Subcellular localisation of cherry leaf roll virus coat protein and genomic RNAs in tobacco leaves.
    Article Snippet: The in vivo subcellular location of the coat protein and RNAs of cherry leaf roll nepovirus (CLRV) was studied in infected tobacco plants by two different approaches and it was correlated with the cytopathic structures induced by the virus.. Subcellular fractions were obtained by differential centrifugation, visualised by electron microscopy and analysed for their viral RNA and coat protein content by Northern blot and Western blot analysis, respectively.. Results indicate that viral RNAs accumulated preferentially at the microsomal fraction.



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